We often tend to use gel elution kits offered by various companies to get our DNA fragment out of agarose gels for cloning and other molecular biology processes. The protocol for eluting DNA using commercial kits is time consuming (even more if u wish to elute multiple bands) and involves toxic chemicals. Here I would to bring to your notice an efficient substitute for gel elution kits–’Freeze ‘N Squeeze’ columns from Bio-RAD.
DNA gel extraction spin columns provide a quick and effective means to purify double-stranded DNA fragments from TAE- or …

The diversity among various life forms on our planet is immense and one reason for their flourishing could be fusion of two prokaryotes, claims a latest report by James A. Lake. This hypothesis which provides new insights into prokaryotes and the evolution of life, was published in latest issue of journal Nature.
The prokaryotes are a group of organisms that lack a cell nucleus , or any other membrane-bound organelles. They differ from the eukaryotes, which have a well defined membrane bound nucleus containing the genetic material DNA. In Prokaryotes ,DNA …

It looks like primates are ruling the science world these days, last week it was famous primate fossil “IDA” made headlines and now Japanese scientists succeeded in generating transgenic primate marmoset . This research might go a long way in use of Marmosets as an alternative disease model to rhesus macaques.Erika Sasaki and her colleagues at the Central Institute for Experimental Animals in Kawasaki injected viral vectors with green fluorescent protein (GFP) into 91 marmoset embryos and successfully shown passing of a transgene a primate to its offspring.Transgenic …

Long wait is finally over ,The draft of the complete genome of the soya bean (Glycine max) has been released by the Joint Genome Institute and its associates at the International Conference on Legume Genomics and Genetics in Puerto Vallarta, Mexico. Soya bean is pretty popular as crop plant for humans and animal food crop, which is rich in oil and protein.Another important property which makes Soya immensely useful is the its use as a feedstock for bio diesel — in which vegetable oil is chemically modified to power diesel …

The genome sequence of woolly mammoth,an extinct species of Elephant is almost complete.This species of Elephant was adapted to live in cold and was found in Tundra region.Scientists from Penn state university have sequenced four billion DNA bases using next-generation DNA-sequencing instruments and a novel approach that reads ancient DNA highly efficiently.Stephan C. Schuster, Penn State professor of biochemistry and molecular biology believes that the genome size of this extinct mammoth might be similar to that of modern day’s African Elephant genome.
The sequencing of nuclear genome of extinct animal …

Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified in rather simple manner,thanks to Kerry Mullis invention way back in 1983. Cell free DNA amplification can be performed in many different ways ,which can be used for identifying and manipulate DNA, detect infectious organisms, also helps in finding mutations, in human genes and numerous other tasks.
“lets you pick the piece of DNA you’re interested in and have as …

Yesterday while loading my PCR samples suddenly a thought struck,why is it always we run our DNA samples in horizontal manner and not horizontal like one employed for proteins.I discussed this stuff with my friends and googled the net out of curiosity.Below is some of the information regarding electrophoresis of DNA and Proteins samples.
Background:
Gel electrophoresis is a technique used for the separation of deoxyribonucleic acid, ribonucleic acid, or protein molecules using an electric current applied to a gel matrix.In most cases the gel is a crosslinked polymer whose composition and …