New site-specific transgenic RNAi library for Drosophila
Vienna Drosophila Research Center (VDRC) recently announced availability of a new large-scale collection of transgenic RNAi lines. These lines have been created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This landing site (VIE-260b) was selected from an initial set of 59candidate landing sites, based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats of typically 300-700bpthat were designed to reduce the risk of off-target effects.
At present, 8,013 lines (one line per gene) are available through the Vienna Drosophila RNAi Center (stockcenter.vdrc.at) and number is expected to reach 10,000 lines available by the end of the year.
Together with the original P element library, still available through the VDRC, this new library should provide a powerful and comprehensiveresource for your transgenic RNAi experiments in Drosophila.
Web Page : http://stockcenter.vdrc.at/
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