How to prepare Drosophila embryos for live cell imaging ?
Its no secret that GFP based time lapse live-imaging is a powerful technique for studying different dynamic processes like tissue morphogenesis ,cell death or cell adhesion. The important aspect which needs to be taken care for live cell imaging to minimize detrimental effects such as dehydration and hypoxia. Recently authors Bruce H. Reed, Stephanie C. McMillan, and Roopali Chaudhary reported a novel technique called hanging drop protocol. The main advantage using hanging drop protocol is that it results in excellent viability of embryos during live imaging and does not require that embryos be compressed live ,which was a major concern in previously used protocols for live imaging. On the web page of JoVE a well narrated video is also available which describes in detail how to collect and prepare Drosophila embryos for live imaging using the hanging drop protocol.
Reference:
The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
Bruce H. Reed, Stephanie C. McMillan, Roopali Chaudhary
JoVE. 25. http://www.jove.com/index/details.stp?id=1206, doi: 10.3791/1206
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